PCI Bridge / Expansion in x86

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Hello  Gurus ,

I am having PCI dataacquistion card which are capable of Bus
in my system .
I only have one PCI Port .

1) Now  if i use PCI expansion card and get many ports ,
does it effect the ability of my PCI dataacquistion devices to do BUS
Mastering ?

2) Is there a limit to the number of Ports i can get via expansion
cards , assuming bandwidth
related issues are taken care of .

3) If the Latest Intel boards have 3 ports , will they all be capable
of handling Bus Mastering,
by the PCI daq devcies .

Appreciate your reply .

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