Hi,
Here is my new crazy idea to combine single or double layer circuits's with microfluidics. It uses two small mirrors, or optionally one mirror and one pane of clear glass (for single sided circuits).
First the microfluidic channels and connectors are milled (or CO2 laser cut etc) into one of the mirrors and then the circuit traces and pads are milled/engraved into the back of the mirror onto the silver layer (ie with eagle cad and pcbgcode and then the components are soldered on.
There can be LED's, lasers, photodiodes etc mounted to view the microfluidic channels, also areas of the silver layer can be milled to create electrostatic or EM fields near the microfluidic channels.
Once the two mirrors or mirror and glass are ready, they are placed glass on glass and permanently attached together, ie with a dilute solution of KOH as described by Phil Hobbs.
For two layer circuits, vias through the two pieces of glass have to be milled or CO2 laser cut, and then jumper wires inserted to attach the two layers.
Also just for making normal PCB's, small mirrors are maybe good, they are $1.25 at the dollar store for a 5" square one, and that is not much more than copper clad FR4 I guess, but the silver metal layer is very thin so only good for low current PCBs I guess.
cheers, Jamie