The Romans? Man, you _are_ old :-)
Ok, then: The tougher category is 80V for 100msec, followed by 48V for one second. Three times at 10sec intervals. Depending on what it is the circuit can pause, but ideally it should just continue to work through these episodes. Throw in 600V spikes as well but they are brief and thus easy. So?
Remember, you've got 15 pennies to play with, no more. And if you use lots of parts then you are charged a pick-and-place tax.
One electronic board but not at liberty to tell.
Nothing, assuming big rigs don't count here. However, some of my 12V designs are allowed to be run on vehicles and on ships (and do so nicely).
I've got tons designs of intra-body stuff. But all this doesn't matter. You said using TVS is like making simple problems difficult so let's not deviate here. How do you solve the problem you snipped away, sans TVS, for less than 15c in parts?